BPB Reports

Paper Details

BPB Reports
Vol. 4 No. 1 p.6-11 2021
Regular Article
Sorghum (Sorghum bicolor (L.) Moench) Extract Enhances Thiazolidinedione-Induced 3T3-L1 Preadipocyte Differentiation but Inhibits Adipogenic Genes
  • Atsuko Masumi (Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences Aomori University / amasumi@aomori-u.ac.jp)
Makoto Sakurai 1) , Ikumi Aizu 1) , Marina Tonosaki 1) , Yuko Oba 1) , Mei Nagata 2) , Katsuyoshi Kamiie 2) , Atsuko Masumi 1)
1) Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences Aomori University , 2) Department Molecular Biology, Faculty of Pharmaceutical Sciences Aomori University
Received: November 20, 2020;   Accepted: January 17, 2021;   Released: January 28, 2021
Keywords: sorghum extract, 3T3-L1 cells, adipogenesis, insulin sensitivity

Sorghum bicolor (L.) Moench is known as a healthful food. We examined whether a water-soluble sorghum extract (SE) from S. bicolor has an anti-diabetic effect through a mechanism that improves insulin sensitivity or anti-adipogenesis. Although the treatment of SE did not affect the adipogenesis of 3T3-L1 adipocytes induced by isobutyl methylxanthine/dexamethasone/insulin (MDI), it significantly enhanced MDI/thiazolidinedione (TZD)-induced adipogenesis in 3T3-L1 adipocyte differentiation. Real-time polymerase chain reaction analysis showed that treatment with SE reduced the expression of adiponectin, adipocyte protein 2 (aP2), and resistin in 3T3-L1 adipocyte cells. SE suppressed the expression of transcription factors, peroxisome proliferator-activated receptor γ (PPARγ), and CCAAT enhancer-binding protein α in both MDI- and MDI/TZDs-treated 3T3-L1 adipocytes. SE treatment reduced tumor necrosis factor α protein in cell lysates from MDI-induced 3T3-L1 adipocytes but not those induced by MDI/TZD. Our results suggest that SE can serve as an effective food source that improves insulin sensitivity and an anti-obesity agent.