BPB Reports

Paper Details

BPB Reports
Vol. 3 No. 1 p.22-27 2020
Regular Article
Role of Metallothionein in Transcriptional Regulation by Metal-Responsive Element-Binding Transcription Factor 1
  • Yasumitsu Ogra (Graduate School of Pharmaceutical Sciences, Chiba University / ogra@chiba-u.jp)
Yu-ki Tanaka , Yoshiaki Futami , Yasunori Fukumoto , Noriyuki Suzuki , Yasumitsu Ogra
Graduate School of Pharmaceutical Sciences, Chiba University
Received: November 15, 2019;   Accepted: December 16, 2019;   Released: February 05, 2020
Keywords: metallothionein, MTF-1, MRE, cadmium, zinc

Owing to the high metal binding affinity of metallothionein (MT), newly synthesized MT was speculated to attenuate the activity of metal-responsive element (MRE)-binding transcriptional factor 1 (MTF-1) by removing Zn from the activated MTF-1. To investigate the potential role of MT in the inactivation of MTF-1, we examined the transcriptional levels of reporter and endogenous MRE-dependent genes using mouse embryonic fibroblasts (MEFs) established from MT-knockout (KO) and wild-type (WT) mice. The activation of MTF-1 by the Cd exposure of MT-KO MEFs was sustained for 12 h, whereas that of MT-WT MEFs showed the rapid attenuation. Consequently, MT was found to negatively regulate MTF-1 activity, which can control the expression of MT itself.