Paper Details
- Naoya Koizumi (Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University / koizumi@ac.shoyaku.ac.jp)
1) Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University , 2) Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University , 3) Laboratory of Hepatocyte Regulation, National Institutes of Biomedical Innovation, Health and Nutrition , 4) Global Center for Medical Engineering and Informatics, Osaka University , 5) PRESTO, Japan Science and Technology Agency , 6) Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), Osaka University
Fiber is an adenovirus (Ad) capsid protein that binds to coxsackievirus and Ad receptor. It is secreted by Ad-infected cells in the early infection stage, and it increases the permeability of the epithelial cells. Accordingly, fiber may facilitate the apical escape of Ad particles from the basolateral side in Ad-infected cells. However, its behavior in the Ad-infected cells remains unclear. Therefore, we investigated the behavior of fiber in the Adinfected cells by fluorescence microscope analysis. Results showed that a higher proportion of fiber molecules were present in the apical side compared with that in the basolateral side, and electrical resistance, which represents cell–cell adhesion, remained unaffected in the Ad-infected cells. Furthermore, the association between fiber secretion and membrane damage was analyzed using annexin V and propidium iodide staining. We observed that fiber was distributed to the membrane surface without membrane damage. In addition, fiber distribution occurred in Ad-infected cells as well as in fiber-expressing cells. Therefore, fiber can distribute itself to the cell surface, and it plays a novel role in Ad infection. Further investigation of fiber distribution would be useful to completely elucidate Ad infection mechanism and develop antiviral strategies for Ad.
