BPB Reports

Paper Details

BPB Reports
Vol. 2 No. 5 p.86-90 2019
Regular Article
Method Validation for the Determination of Phthalates in Indoor Air by GC-MS with Solid-Phase Adsorption/Solvent Extraction using Octadecyl Silica Filter and Styrene–Divinylbenzene Copolymer Cartridge
  • Hideto Jinno (Meijo University / jinno@meijo-u.ac.jp)
Toshiko Tanaka-Kagawa 1) , Ikue Saito 2) , Aya Onuki 2) , Maiko Tahara 3) , Tsuyoshi Kawakami 3) , Shinobu Sakai 3) , Yoshiaki Ikarashi 3) , Shiori Oizumi 4) , Masahiro Chiba 4) , Hitoshi Uemura 5) , Nobuhiko Miura 1) , Ikuo Kawamura 1) , Nobumitsu Hanioka 1) , Hideto Jinno 6)
1) Yokohama University of Pharmacy , 2) Tokyo Metropolitan Institute of Public Health , 3) National Institute of Health Sciences , 4) Hokkaido Institute of Public Health , 5) Kanagawa Prefectural Institute of Public Health , 6) Meijo University
Received: October 02, 2019;   Accepted: October 09, 2019;   Released: November 01, 2019
Keywords: phthalic acid esters, indoor air, solvent extraction, octadecyl silica-gel filter, styrene divinylbenzene copolymer cartridge

This study proposes and evaluates a precise and labor-saving method for quantifying phthalic-acid esters (PAEs) in indoor air based on solid-phase extraction. Three different adsorbents were evaluated; i.e., two types of octadecyl silica (ODS) filter and a styrene–divinylbenzene (SDB) copolymer cartridge. Calibration curves for five PAEs [diethyl phthalate (DEP), diisobutyl phthalate, di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), and di(2-ethylhexyl) phthalate (DEHP)] were created using an internal standard (DBP-d4). Values of the coefficient of determination (R2) indicated good linearity of the calibration curves (R2 > 0.9953). Among the three adsorbents, the SDB cartridge was easiest to handle because it can be used without cleaning and has the lowest blank value. The recovery of deuterated PAEs (DEP-d4, DBP-d4, BBP-d4, and DEHP-d4) did not differ significantly among the three adsorbents; values were consistently > 89.7% for an air volume of 2.88 m3. During simultaneous indoor air sampling, PAE concentrations were very similar for the three adsorbents. Interlaboratory validation studies were conducted in five laboratories to validate the proposed method for two PAEs (DBP and DEHP). The mean recoveries of the two PAEs added to two types of adsorbent were 91.3–99.9%, the reproducibility relative standard deviations (RSDR) were 5.1–13.1%, and the Horwitz ratio (HorRat) values were 0.31–0.79. The proposed method using solid-phase extraction with two types of adsorbents provides accurate estimates of PAEs in ambient air.