BPB Reports

Paper Details

BPB Reports
Vol. 5 No. 5 p.95-98 2022
Anti-Obesity Dendritic Cell Vaccine Therapy Targeting Neovascular Vessels in High-Fat-Diet-Fed Mice
  • Naoki Utoguchi (Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University / utoguchi@ac.shoyaku.ac.jp)
Yoshikazu Sawaguchi 1) , Keiichi Hirata 2) , Ryo Suzuki 2) 3) , Kazuo Maruyama 3) 4) , Naoki Utoguchi 5)
1) Faculty of Biomedical Engineering, Toin University of Yokohama , 2) Laboratory of Drug and Gene Delivery Research, Faculty of Pharma-science, Teikyo University , 3) Advanced Comprehensive Research Organization (ACRO), Teikyo University , 4) Laboratory of Theranostics, Faculty of Pharma-science, Teikyo University , 5) Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
Received: May 21, 2022;   Accepted: August 05, 2022;   Released: August 30, 2022
Keywords: obesity, vaccine, neovascular, endothelial cell, dendritic cell

Obesity is a severe disease that causes diabetes, fatty liver, hypertension, and arteriosclerosis. It involves adipocytokines such as tumor necrosis factor-α, leptin, and resistin.The hypertrophic adipocytes induce the secretion of these adipocytokines. Regulating the growth of adipose tissue is an extremely important approach to prevent these diseases. Hypertrophy of adipose tissue is closely associated with angiogenesis, wherein neovascular vessels supply nutrients.Previous studies have shown that angiogenesis inhibitors can prevent the development of obesity in mice. In this study, we report an anti-obesity therapy targeting the neovascular vessels of adipose tissue via endothelial cell (EC) vaccination. EC vaccine was prepared using EC extract antigen, which was pulsed to dendritic cells, and was administered thrice intradermally. The anti-obesity effect was evaluated in high-fat diet-fed obesity model mice. EC vaccination significantly suppressed the increase in body weight and hyperplasia of adipocytes and improved the accumulation of hepatic lipid droplets in these mice. Adipose tissue-induced vascular-targeted vaccine therapy is a potential novel approach for obesity.