The gastrointestinal absorption of heavenly blue anthocyanin derived from morning glory (Pharbitis nil L.) was examined in rats. Ingested heavenly blue anthocyanin was directly absorbed from the gastrointestinal tract and detected in its original polyacylated form in rat blood plasma. The maximum plasma concentration and area under the plasma concentration curve during 8-h post-administration after a single oral dose of 0.0569 mmol/kg heavenly blue anthocyanin were 0.143 ± 0.023 μM and 23.30 ± 3.76 μM·min, respectively. Heavenly blue anthocyanin, which contains asymmetrical branched chains with glucosyl terminals, was absorbed at a similar level to that of total ternatin. By contrast, the plasma amount of heavenly blue anthocyanin was approximately 6-fold higher than that in a previous report of peonidin 3-O-β-(6''-O-caffeoyl)-sophoroside-5-O-β-D-glucopyranoside with an attached caffeoyl terminal on 6''-position.