BPB Reports

Paper Details

BPB Reports
Vol. 3 No. 5 p.166-169 2020
Report
Metal Responsive Transcription Factor-1 is Selectively Expressed among the Mouse Testicular Cells
  • Yoichi Osada (Laboratory of Molecular Environmental Health, Department of Life and Health Sciences, Faculty of Pharma-Sciences, Teikyo University / yoosada@pharm.teikyo-u.ac.jp)
Yoichi Osada , Tae Shimoyama , Fuminori Otsuka
Laboratory of Molecular Environmental Health, Department of Life and Health Sciences, Faculty of Pharma-Sciences, Teikyo University
Received: August 15, 2020;   Accepted: October 14, 2020;   Released: October 26, 2020
Keywords: zinc regulatory transcription factor, spermatogenesis, zinc-dependent regulation
Abstracts

Metal responsive transcription factor-1 (MTF-1) is ubiquitously expressed in various tissues, and is thought to be an intracellular zinc sensor that regulates transcriptional activation of zinc responsive genes. We investigated the distribution of MTF-1 in the mouse testis, where zinc plays an essential role in spermatogenesis. By performing immunohistochemical analysis of the ddY mouse testis using the anti-MTF-1 antibody, we observed a donut-shaped staining pattern in the seminiferous tubules in the region proximal to the lumen, where spermatocytes primarily localize. A similar staining pattern was obtained using in situ hybridization to detect MTF-1 mRNA. Furthermore, we confirmed that MTF-1 could not be detected at both the protein and mRNA levels in the premature 20-d-old mouse testis, where spermatocytes are thought not to have been formed in the seminiferous tubules yet. These lines of evidence strongly indicated the selective expression of MTF-1 in mouse spermatocytes and suggested that MTF-1 played a role in a certain stage of spermatogenesis.